FIG. 3.

AcMNPV DNA synthesis is blocked upon silencing of the replicative lefs. SF21 monolayers were transfected with the indicated dsRNAs and infected 24 h later with AcMNPV wt/lacZ (MOI = 10). At 12, 24, and 48 h after infection, the cells were collected, lysed, and extracted for total DNA. AcMNPV DNA levels were quantified by real-time PCR as described in Fig. 2. Values are reported as the level of viral DNA ± the standard deviation from six different plates compared to that of control egfp dsRNA-transfected, wt/lacZ-infected cells. Solid and cross-hatched bars depict values obtained upon transfection of dsRNA specific for replicative and nonreplicative lefs, respectively.