FIG. 5.

AcMNPV-induced apoptosis is blocked upon silencing of replicative but not nonreplicative lefs. (A) Apoptotic cytolysis. SF21 monolayers were transfected with the indicated dsRNAs and inoculated (+virus) 24 h later with apoptosis-inducing AcMNPV p35-deletion mutant vΔ35K/lacZ (MOI = 10). Representative photographs (×250 magnification) taken 26 h after infection are shown. (B) Cell survival. Intact SF21 cells that were mock-infected (mi) or infected (+virus) 26 h earlier with vΔ35K/lacZ as described for panel A were counted by using computer-aided microscopy. Values shown represent the percent ± the standard deviation of surviving cells averaged for three independent plates and compared to that of mock-infected, egfp dsRNA-transfected cells. The results of a representative experiment are shown.