FIG. 7.

In vivo replication of MDVs carrying B-ZIP mutations in Meq. Shown is the replication of MDV in the PBL of line P (A) and RIR (B) chickens infected with the pRB-1B BAC viruses as described in the legend to Fig. 5. A qPCR method (1) was used to determine the copy number of MDV genomes in DNA extracted from PBL samples taken at the times indicated throughout the experiment. Samples were taken from the same six birds from each group throughout the experiment. The mean genome copy number for the six birds is shown at each time point, with the bars representing the 95% confidence interval. (C) Analysis similar to that shown in panel A using DNA extracted from the feather tips of infected line P birds. The standard error of the mean for each group is shown.