Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
53BP1 association with Zta in vivo. HEK 293-BZLF1-KO cells were transfected with pCDNA3 alone or with pCDNA3 HisZta using Effectene transfection reagent. Raji cells were transfected with the same constructs using Amaxa transfection technology. After 48 h, cells were lysed and nickel agarose-associated proteins purified. Samples were analyzed by Western blotting for 53BP1 (top) and Zta (bottom), as indicated. Proteins then were detected by chemiluminescence.
