FIG. 4.

C-terminal region of Zta is important in 53BP1 binding in vitro. (A) Schematic diagram showing part of the Zta sequence with the location of the Zta termination mutants indicated above the zipper and C-terminal regions. The zipper region is shown by a gray box, and the carboxyl-terminal region is shown by the black box below the sequence. (B) The indicated in vitro-translated proteins were generated in wheat germ extract. Zta and its termination mutants were analyzed by SDS-PAGE on a 12% gel and quantitated after detection using phosphorimaging. (C) Equivalent quantities of in vitro-translated proteins, indicated above each lane, were incubated with GST-53BP1-BRCT-Sepharose or GST, as indicated, and the Zta that associates is shown.