FIG. 7.

53BP1 contributes to EBV genome replication. siRNA duplexes directed against 53BP1 or a control (con) duplex (10 nM) were introduced into LCL#3 cells for 4 days. (A) The effect of each siRNA on 53BP1 protein expression and the expression of the protein encoded by the housekeeping gene GAPDH were monitored by the Western blot analysis of cell extracts with specific antibodies as indicated. (B) Supernatant from the cells was harvested, and the amount of EBV genome in virions was determined by the qPCR detection of the EBV genome.