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. 2009 Aug 19;83(21):11283–11297. doi: 10.1128/JVI.00756-09

FIG. 3.

FIG. 3.

Virion-associated Vpr triggers PS exposure. (A) Jurkat cells were mock infected or infected with Vpr(+)trans or Vpr(−) viruses at an MOI of 0.5. Fifty hours postinfection, the cells were stained with A5PE and analyzed by flow cytometry to generate dot plots. The fluorescence intensities of EGFP and A5PE are represented on the x axis and the y axis, respectively. Uninfected cells treated with 1 μM staurosporine (STS) for 4 to 6 h were used as a positive control for the assay. The plots shown are representative of one of four independent experiments. NLEGFP virus infection and subsequent staining with A5PE were used as a reference. (B) The ratio of the percentage of EGFP+ A5PE+ cells to that of EGFP+ A5PE− cells was determined for each viral infection from the dot plots in panel A and was plotted on a bar graph. The results are shown as means plus standard deviations. Note that the ratio indicated for mock infection is the ratio of the percentage of A5PE+ cells to that of A5PE− cells, which was used as an internal control.