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. 2009 Aug 26;83(21):11244–11253. doi: 10.1128/JVI.01413-09

FIG. 6.

FIG. 6.

Expression of PrP mRNA in microglia and astroglia. Expression of mRNA encoding PrP and five other genes in microglia (MG) and astroglia (AG) was determined using quantitative real-time reverse transcriptase PCR. Data are shown as the percent difference relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression for the following genes: PrP (A), GFAP (B), F4/80 (C), CCR1 (D), CCR5 (E), and CCR2 (F). Two different litters of mice were analyzed in separate experiments with six wells of PCR per experiment. Statistical analysis was performed by a one-tailed Mann-Whitney test (***, P < 0.001). Microglia expressed approximately 20-fold-less PrP mRNA than astroglia did. Microglia expressed significantly higher levels of chemokine receptors CCR1, CCR2, and CCR5 than astroglia did. Each symbol shows the amount of mRNA from one mouse, and the black bar shows the mean value.