Table 2. Segregation of ASHH2/ashh2 in reciprocal crosses with wt.
Female X Male | ASHH2/ASHH2 | ASHH2/ashh2 | ashh2/assh2 | N | Ta | TEb | Δc | EXP homod |
ASHH2/ashh2-1 selfed | 37% | 55% | 8% | 62 | N.D. | N.D. | N.D. | 9% |
ASHH2/ashh2-1 x Col | 61% | 39% | 0% | 129 | 0.78 | 0.58 | 21% | N.D. |
Col x ASHH2/ashh2- 1 | 63% | 37% | 0% | 142 | 0.74 | 0.59 | 20% | N.D. |
Transmission of mutant allele (T) was calculated as T(observed)/T(expected).
Transmission efficiency (TE) calculated as observed number of transmitted mutant alleles (TEobs)/expected number of transmitted mutant alleles (TEexp). TEexp for the mutant allele correspond to the observed number of WT alleles transmitted in the reciprocal cross (Howden et al., 1998).
Expected frequency of defective male or female gametophytes in a ASHH2/ashh2 plant. Δ = 0.5 (1-TE)×100%.
Expected frequency of homozygous plant calculated from TE(obs)female and TE(obs)male.