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. Author manuscript; available in PMC: 2010 Mar 24.
Published in final edited form as: Neuron. 2009 Sep 24;63(6):788–802. doi: 10.1016/j.neuron.2009.08.006

Figure 5.

Figure 5

ban activity is broadly distributed in late larval stages but is dispensible in neurons for dendrite scaling.

(A) A control miRNA sensor is ubiquitously expressed throughout larval development. Anti-GFP immunostaining of a control miRNA sensor 3rd instar fillet reveals expression in muscle (arrow), epithelia (open arrowhead), and PNS neurons (bracket), which are labeled by anti-HRP immunoreactivity in magenta (A’). Scale bars are 50 μm for (A-E).

(B-F) ban sensor expression in larvae. ban sensor GFP expression is not detectably expressed in 3rd instar larvae (B). In (B’) ban sensor expression (green) and HRP (magenta) are shown. (C-D) Live imaging of the ban sensor in larvae using identical microscope settings at 48 hr AEL (E) and 72 hr AEL (F) shows a marked down-regulation of GFP expression over this interval. (E) ban sensor expression remains high in ban mutant larvae, even at 96 hr AEL, demonstrating that the down-regulation of the sensor seen in (D) and (E) depends on ban function. (F) Live imaging of the ban sensor in ban△1 ddaC MARCM clones to monitor ban activity in clones. The ban sensor (green) and the CD2-Cherry used to positively label the clone (magenta) are shown.

(G-I) ban is dispensable in neurons for dendrite scaling. Control (G) or ban△1 (H) ddaC MARCM clones in dissected 3rd instar larval fillets. (I) Quantification of coverage index for control or ban△1 MARCM clones. For all categories n > 5.