Table 1.

Effect of CaCl₂ extraction on bone morphogenesis in implants of bone matrix four weeks after implantation

Solution	mm3 new bone/6 mm3 preimplanted matrix*	mg ash/gm preimplanted matrix
0.9% NaCl	5.0	520 ± 51
0.1 M CaCl2	5.0	550 ± 48
0.2 M CaCl2	5.0	495 ± 32
0.6 M CaCl2	5.0	500 ± 58
1.0 M CaCl2	4.5	480 ± 38
2.0 M CaCl2	3.0	325 ± 29
6.0 M CaCl2	2.0	150 ± 12
6.0 M CaCl2 (tissue diced, 1 day in solution)	0	0
0.6 M CaCl2 (tissue diced, 1 day in solution)	5.0	502 ± 45
0.6 M CaCl2 (tissue diced, 4 days in solution)	4.5	490 ± 42
0.6 M CaCl2 (tissue diced, 9 days in solution)	4.0	395 ± 40
0.6 M CaCl2 (tissue diced, 25 days in solution)	0	0

* Based on correlated radiographic and quantitative histologic observations.