Table 2.

Bone morphogenetic activity of demineralized matrix treated with enzymes

Treatment of the preimplanted matrix	mm3 new bone/6 mm3 preimplanted matrix	mg bone ash/gm preimplanted matrix
Control (no treatment)	5.0	520 ± 51
α-Amylase in phosphate buffer, pH 5.4, at 25 C, 90 hr	5.0	490 ± 40
α-Amylase in phosphate buffer, pH 5.4, at 2 C, 90 hr	5.0	500 ± 10
Phosphate buffer without amylase, at 25 C, 90 hr	5.0	510 ± 40
Phosphate buffer without amylase, at 2 C, 90 hr	5.0	515 ± 20
Trypsin in phosphate buffer, pH 7.6 at 15 C, 8 hr	0.5	50 ± 5
Trypsin in phosphate buffer, pH 7.6, at 15 C, 24 hr	0	0
Trypsin in phosphate buffer, pH 7.6, at 15 C, 48 hr	0	0
Trypsin in phosphate buffer, pH 7.6, at 15 C, 96 hr	0	0
Phosphate buffer without trypsin, at 15 C, 96 hr	0	485 ± 20