Figure 1.

Gβ interacts with Axin. (A) Co-immunoprecipitation of myc-Axin and Flag-Gβ₂. Myc-tagged Axin and FLAG-tagged Gβ₂ were transfected into HEK293T cells. The lysates were first subjected to immunoprecipitation (IP) using anti-Myc antibody, followed by western blotting (WB) using the antibodies indicated on the left side. β-catenin and GSK3β were used for positive controls. (B) Myc-tagged Axin with FLAG-tagged isoforms of Gβ or EYFP were transfected into HEK293T cells. Immunoprecipitation followed by western blotting was performed to test the specificity of interaction between Axin and isoforms of Gβ. (C) Deletion constructs of Axin with HA-tagged Gβ₂ and Gγ₂ were transfected into HEK293T cells and the expression was confirmed by western blot (left panel). Immunoprecipitation with anti-Myc antibody followed by western blotting was performed to examine specific interaction (right panel). (D) Schematic diagram of deletion constructs and summary of interaction between Axin and Gβ₂ was depicted.