Figure 6.

TSAd is required for anti-CD3 Ab + laminin (LM)-induced FAK phosphorylation. (A) D1.1 cells were transfected with pSUPER or plasmids expressing two types of hTSAd-shRNA (siA or siB) and incubated for 30 min on plates coated with anti-CD3 Ab + laminin. Cell lysates were subjected to Western blotting with anti-p-FAK or anti-FAK Ab. (B) TSAd is not involved in CD3 + laminin-stimulated ERK1/2 phosphorylation. The same cell lysates were subjected to Western blotting with anti-p-ERK1/2 or anti-ERK1/2 antibody. (C) As controls for the level of TSAd, the same cells were analyzed by Western blotting with anti-TSAd Ab.