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| Sites | Composition | Root | Preferred | Inferred Substitutions | |
| Human I-v (100 sequences) | 143 | V99T1 | V | V | T→1V |
| 156 | G90R9E1 | R | R | G→1E, G→7R | |
| 158 | N96K4 | N | K | N→4K | |
| 190 | D67N25V8 | D | V | D→16N, D→4V | |
| 193 | A52T48 | A | T | A→6T | |
| 197 | T82K18 | T | T | K→2T | |
| Human 1933∼1979 (32 strains) (egg-adapted) | 225 | G23D9 | D | D | D→1G, G→6D |
| Human 1948∼1979 (17 strains) (egg-adapted) | 225 | G13D4 | G | D | G→4D |
