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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2009 May 15;47(3):312–320. doi: 10.1016/j.freeradbiomed.2009.05.012

Figure 4. Effect of Gpx4 on diquat-induced Smac/DIABLO release from liver mitochondria.

Figure 4

Tg(GPX4+/0) and Gpx4+/− mice and their corresponding Wt littermates were either untreated or treated with diquat. Smac/DIABLO release was determined by measuring the levels of Smac/DIABLO in the cytosol using Western blotting as described in the Experimental Procedures. Panel A: Photograph of a representative Western blot showing Smac/DIABLO release into the cytosol from the Tg(GPX4+/0) mice and their Wt littermates. Panel B: Quantification of Smac/DIABLO release in the Tg(GPX4+/0) mice (solid bars) and their Wt littermates (open bars) as determined from the Western blotting. Panel C: Photograph of a representative Western blotting showing Smac/DIABLO release into the cytosol from the Gpx4+/− mice and their Wt littermates. Panel D: Quantification of Smac/DIABLO release in the Gpx4+/− mice (shaded bars) and their Wt littermates (hatched bars) as determined from the Western blotting. All values are mean ± SEM of data obtained from 3–4 mice. The asterisks indicate the differences that are significantly different.