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. Author manuscript; available in PMC: 2010 Jan 13.
Published in final edited form as: Curr Biol. 2008 Dec 24;19(1):37–42. doi: 10.1016/j.cub.2008.11.033

Figure 1. LKB1 localizes at adherens junction level in polarized epithelial cells.

Figure 1

(A) Polarized Caco-2 stained for LKB1 (green) and E-cadherin (red). (B) xz projection of polarized Caco-2 cells stained for LKB1 (green) and villin (red) or (C) for LKB1 (green) and ZO-1 (red). (D) Polarized Caco-2 cells were fractionated as described in “experimental procedures” and analyzed for indicated proteins. Results are representative of two independent experiments. (E) MDCK clones stably knocked down for LKB1 (cl) by shRNA or control construct (shLuc) transiently transfected by either GFP alone or GFP-LKB1wt were analysed by Western blotting for indicated proteins. (F) AMPKp-172/total AMPK ratio (black bar) were determined and plotted with endogenous (endo, white bar) and exogenous (exo, grey bar) LKB1, all relative to control. Values are means ± S.D., (n=2). (G) Polarized MDCK shLuc and shLKB1 cl 14 were stained for LKB1 (green), E-cadherin (purple) and villin (red). Bars, 10µm.