Table 4. PCR primers used in the present study.

Name	Nucleotide sequence (5′ to 3′)	Use
CB23	ACTTTACAGTTGCTAGATAAGC	for PCR of Southern Probe A
CB24	AGCAGGTTACTTGTCCAGTC	for PCR of Southern Probe A
CB107R	AATTCTTTAGACCCTTCTTTGTTT	for PCR of Southern Probe D
CB108F	TGTCAATAATGCAGCTGATGATGGGCTTTCATTGTCTTCTCATTCTTAGATGAGCCCATGGATTCTAC	for PCR of Southern Probe D
CB156F	CAA GCAG GGG AAG GAG GC	PCR detection ATE1-floxON
CB157R	TTC AGG AGT TAG CCA TTG CC	PCR detection ATE1-floxON and ATE1-floxOFF
AK49	GGT ATT TGC TGC CGT CCT TTG GTG GT	PCR detection of ATE1-null
YT641	CTG TTC CAC ATA CAC TTC ATT CTC AG	PCR detection of ATE1-null
AK83-Cbfix	CTG GAG ACA AAG CCC CAG CCA GAC	PCR detection of ATE1-null
Cre-1	GTT CGC AAG AAC CTG ATG GAC A	PCR detection of Cre gene
Cre-2	CTA GAG CCT GTT TTG CAC GTT C	PCR detection of Cre gene
CB159R	AC TGT AGA ATC CAT GGG CTC	PCR detection wild type ATE1
CB160F	ACA GCA TAA GTG AGA CAC TCA	PCR detection wild type ATE1
CB110F	GTT TGT GTC ACC ACT CCT ACC	PCR detection ATE1-floxOFF
oIMR0042	CTA GGC CAC AGA ATT GAA AGA TCT	PCR detection IL-2 control
oIMR0043	GTA GGT GGA AAT TCT AGC ATC ATC C	PCR detection IL-2 control
AgRP-for	GCGGAGGTGCTAGATCCA	RT-PCR
AgRP-rev	AGGACTCGTGCAGCCTTA	RT-PCR
NPY-for	CTCCGCTCTGCGACACTAC	RT-PCR
NPY-rev	AATCAGTGTCTCAGGGCT	RT-PCR
POMC-for	ACCTCACCACGGAGAGCA	RT-PCR
POMC-rev	GCGAGAGGTCGAGTTTGC	RT-PCR
MCH-for	ATTCAAAGAACACAGGCTCCAAAC	RT-PCR
MCH-rev	CGGATCCTTTCAGAGCAAGGTA	RT-PCR
cyclophilin-for	GGTGGAGAGCACCAAGACAGA	RT-PCR
Cyclophilin-rev	GCCGGAGTCGACAATGATG	RT-PCR
Ucp2-5′	GGGGCGGCCGCATGGTTGGTTTCAAGGCCAC	RT-PCR
Ucp2-3′	GGGGCGGCCGCTCAGAAAGGTGCCTCCCGAG	RT-PCR
Actb-5′	ATGGATGACGATATCGCTGCG	RT-PCR
Actb-3′	GAAGCTGTAGCCACGCTCGG	RT-PCR
Leptin-5′	GGGGCGGCCGCATGTGCTGGAGACCCCTGTG	RT-PCR
Leptin-3′	GGGGCGGCCGCTCAGCATTCAGGGCTAACAT	RT-PCR
Ucp1-5′	GGGGCGGCCGCATGGTGAACCCGACAACTTC	RT-PCR
Ucp1-3′	GGGGCGGCCGCTTATGTGGTACAATCCACTG	RT-PCR