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. 2009 Nov;151(3):1339–1353. doi: 10.1104/pp.109.145987

Figure 5.

Figure 5.

Analysis of the gun1-101 ppi2-2 double mutant obtained by embryo rescue. A to D, Embryogenesis of the gun1-101 ppi2-2 double mutant viewed with a light microscope with a differential interference contrast unit. A, Early heart stage. B, Late heart stage. C, Walking stick stage. D, Mature embryo stage. E and F, Visible phenotypes of ppi2-2 (E) and gun1-101 ppi2-2 (F) plants obtained by embryo rescue. Seedlings were cultured for 2 weeks. Bars = 1 mm. G, Quantitative analysis of gene expression in embryo-rescued (ER) ppi2-2 and gun1-101 ppi2-2 seedlings (2 weeks old). The mRNA levels were analyzed by real-time PCR and normalized to the levels of ACTIN2. The expression level in wild-type seedlings (1 week old) was set to 1. Each bar, plotted here on a log scale, represents the mean of at least three independent samples. Error bars represent 1 sd. Asterisks above the bars indicate significant differences (P < 0.05) between ppi2-2 and gun1-101 ppi2-2, as determined by Student's t test. H, Accumulation of plastid proteins in embryo-rescued seedlings (2 weeks old). Total protein extracts were resolved by SDS-PAGE and probed with antisera against the proteins indicated to the left.