Genetic complementation of the anthocyanin and PA phenotypes of the NF0977 retrotransposon insertion line. A, Pigmentation of hairy roots of the NF0977 line expressing GUS (left) and MtWD40-1 (right). B, qRT-PCR analysis of MtWD40-1 transcript levels in hairy roots of NF0977 expressing GUS or MtWD40-1. C, As above, showing ANS transcript levels. D, As above, showing UGT78G1 transcript levels. E, Anthocyanin levels from NF0977 expressing GUS or MtWD40-1 (three independent lines of each). F, As above, showing insoluble PA levels. G, As above, showing soluble PA levels. FW, Fresh weight.