Figure 5.

lon2 mutants degrade MLS normally but have defects in PTS2-containing protein accumulation and processing. Immunoblots of total protein extracted from wild-type and lon2-1 (A), lon2-2 (B), or deg15-1 (C) cotyledons (12 per lane) from 3- to 8-d-old seedlings. Blots were probed with antibodies raised against the PTS1-containing protein MLS, the PTS2-containing proteins PED1 (thiolase) and PMDH2, and HSC70 as a loading control. The positions of M_r markers (in kD) are indicated on the left; processed and unprocessed PTS2 proteins are marked by one or two asterisks, respectively.