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. 2009 Nov;151(3):1354–1365. doi: 10.1104/pp.109.142505

Figure 8.

Figure 8.

lon2 mutant cotyledon cells import some matrix proteins normally at 4 d old, but matrix protein import is impaired at 8 d. A, Cotyledons from 4- and 8-d-old light-grown Arabidopsis seedlings were immunolabeled with antibodies raised against CAT, PTS2 proteins (PED1 and PMDH2), PTS1 proteins (MLS and HPR), and a membrane-bound peroxin (PEX14). Bar = 10 μm. B and C, Immunoblot of total protein extracted from wild-type, lon2-1, lon2-2, deg15-1, and pmdh1 pmdh2 aerial (eight seedlings, aerial tissue only) or root (16 roots per lane) tissue. Seeds were stratified for 1 d prior to plating. Blots were probed with anti-PED1 (thiolase), PMDH2, and HSC70 antibodies. Positions of Mr markers (in kD) are indicated on the left; processed and unprocessed PTS2 proteins are marked by one or two asterisks, respectively.