Skip to main content
. 2009 Nov;151(3):1294–1303. doi: 10.1104/pp.109.142539

Figure 6.

Figure 6.

Substrate determination for P. alliacea LFS using RP C-18 HPLC. A 20 μL reaction mixture comprised of 1.0 mL of 10 mm phosphate buffer, pH 8.0, 25 μm PLP, 1.5 mm petiveriin (or 0.25 mm petivericin), and 5.7 μg of purified LFS (approximately 100 nm) at several LFS-to-alliinase molar ratios was incubated for 10 min at room temperature and then analyzed by HPLC to reveal what products, if any, were formed. The substrate used in the reaction for A, B, D, E, and F was petiveriin, and the substrate for C was petivericin. A, Only alliinase was present in the reaction; B and C, only LFS was present in the reaction; D, both LFS and alliinase were present in the reaction, and LFS:alliinase molar ratio was 5:1; E, LFS:alliinase molar ratio was 5:2; F, LFS:alliinase molar ratio was 5:3.