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Purification of the LFS from 150 g of P. alliacea roots
| Fraction | Volume | Total Protein | Total Activitya | Specific Activity | Recovery | Purification |
|---|---|---|---|---|---|---|
| mL | mg | nkat | nkat mg−1 | % | fold | |
| Homogenate | 230 | 289 | – | – | – | – |
| 60% (NH4)2SO4 | 20 | 152 | – | – | – | – |
| Anion exchange | 4.0 | 26 | 140 | 5.5 | 100 | 1.0 |
| Hydroxyapatite (1st) | 4.0 | 9.2 | 58 | 6.3 | 40 | 1.1 |
| Hydroxyapatite (2nd) | 2.6 | 2.9 | 23 | 7.9 | 16 | 1.4 |
| Gel filtration |
2.6 |
1.0 |
12 |
12 |
8 |
2.2 |
1 nkat = 1 nmol PMTSO s−1.
