Figure 3.

RT-PCR detection of SVs in Atsmu2-1 and Atsmu2-2. RT-PCR was performed using total RNA from 14-DAG Col-0 (lane 1), smu2-1 (lane 2), and smu2-2 (lane 3) seedlings. Arrowheads indicate the positions of known SVs of At1g09140 (AtSRp30) and At1g07350 (AtTRA2), while asterisks correspond to putative SVs first observed here. Pre-mRNA splicing patterns are shown on the right side, with dark gray boxes and light gray bars between the boxes representing exons and introns, respectively. Arrows depict primers used in each reaction. Ratios of differentially accumulated SVs are shown below the panels.