Figure 4.

TAP-purified proteins detected by antibodies in western blots. Proteins were extracted from Arabidopsis suspension cell cultures overexpressing a TAP-fused polyadenylation-related protein, as depicted at the top of each lane. After tandem purifications, the proteins were separated by 12% SDS-PAGE and then transferred to a PVDF membrane. The proteins on the membrane were detected by antibodies (α) raised against the proteins listed at the left side of the blot. Protein size markers (kD) are listed on the right.