Table II.
Selected metabolites identified by GC-MS in bean nodules from –P- and +P-treated plants
| Metabolite | RI, Expected | RI, sd | Response Ratio −P/+Pa |
|---|---|---|---|
| Amino acids | |||
| Gly | 1,304.5 | −0.17 | −1.4 |
| β-Ala | 1,424.7 | −0.12 | 3.5 |
| Ser | 1,252.9 | 0.09 | −1.2 |
| Asn | 1,665.8 | −0.02 | 2.0 |
| Thr | 1,290.9 | −0.02 | −2.0 |
| 4-Hyp | 1,518.0 | 0.21 | −1.4 |
| Gln | 1,767.6 | −0.05 | −1.5 |
| Leu | 1,151.0 | 0.52 | 1.6 |
| Lys | 1,847.3 | −0.02 | 1.2 |
| Phe | 1,553.1 | 0.15 | 2.4 |
| N compounds | |||
| Putrescine (agmatine)b | 1,737.2 | −0.24 | −1.4 |
| Pipecolic acid | 1,366.6 | −0.16 | −1.6 |
| Picolinic acid | 1,327.2 | 0.20 | 1.5 |
| Spermidine | 2,251.1 | −0.22 | −1.6 |
| Urea | 1,235.6 | 0.06 | −1.4 |
| Organic acids | |||
| Malonic acid | 1,195.0 | 0.30 | 1.4 |
| Tartaric acid | 1,625.9 | 0.03 | −1.7 |
| Malic acid | 1,477.3 | 0.11 | 1.5 |
| 2,4-Dihydroxybutanoic acid | 1,403.6 | −0.08 | −2.1 |
| cis-Aconitic acid | 1,740.5 | −0.02 | 1.6 |
| Citric acid | 1,804.6 | −0.04 | 1.2 |
| Vanillic acid | 1,761.6 | 0.10 | 1.7 |
| Polyhydroxy acids | |||
| Glyceric acid | 1,321.7 | −0.02 | −1.1 |
| Threonic acid | 1,546.4 | −0.04 | 1.4 |
| Galactonic acid-1,4-lactone | 1,877.4 | −0.31 | 1.3 |
| Galactaric acid | 2,032.5 | −0.22 | 1.5 |
| Galactonic acid | 1,984.7 | −0.29 | 1.8 |
| Gulonic acid | 1,951.4 | −0.21 | 2.0 |
| Phosphates | |||
| Glyceric acid-3-phosphate | 1,790.2 | 0.06 | −1.1 |
| Fru-6-P | 2,292.8 | −0.03 | −2.2 |
| Glu-6-P | 2,329.6 | −0.13 | −1.3 |
| Polyols | |||
| Threitol | 1,485.1 | −0.12 | 3.3 |
| Sugars | |||
| Fru | 1,856.2 | −0.24 | −1.1 |
| Man | 1,869.0 | −0.17 | 1.4 |
| Suc | 2,629.6 | −0.28 | 2.2 |
| α,α′-Trehalose | 2,730.1 | −0.26 | 1.6 |
| MSTscd | |||
| [516; 1H-Indole-2,3-dione, 1-(tert-butyldimethylsilyl)-5-isopropyl-, 3-(O-methyloxime)] | 1,691.2 | −0.04 | −2.4 |
| [771; α-d-Methylfructofuranoside (4TMS)] | 1,760.9 | −0.02 | 1.5 |
| [802; Methylcitric acid (4TMS)] | 1,909.57 | −0.06 | 1.2 |
| [965; Gluconic acid, 2,3,4,5,6-pentakis-O-(trimethylsilyl)-trimethylsilyl ester] | 2,002.9 | −0.27 | 1.6 |
| [834; 2-O-Glycerol-β-d-galactopyranoside (6TMS)] | 2,160.3 | −0.04 | −1.5 |
| [926; Galactosylglycerol (6TMS)] | 2,297.2 | −0.12 | 2.4 |
| [802; Gulose (5TMS)] | 2,424.3 | 0.26 | −1.9 |
| [533; Lactose methoxyamine (8TMS)] | 2,878.2 | −0.10 | 1.3 |
| [882; Melibiose (8TMS)] |
3,092.9 |
−0.27 |
2.6 |
a
The response ratio of average –P nodule response compared with average +P nodule response is listed (t test significance of P < 0.05 is indicated by boldface numbers for the response ratio). For ratios lower than 1, the inverse of the ratio was estimated and the sign was changed.
b
Represents the sum of two or more metabolites.
c
Reference substance not yet available.
d
MSTs are characterized by match factor and mass spectral hit.