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. 1964 Aug;88(2):367–373. doi: 10.1128/jb.88.2.367-373.1964

GENE TRANSFER BY F′ STRAINS OF ESCHERICHIA COLI IV.

Effect of a Chromosomal Deletion on Chromosome Transfer

James Pittard a,1, T Ramakrishnan a,2
PMCID: PMC277309  PMID: 14205619

Abstract

Pittard, James (Yale University, New Haven, Conn.), and T. Ramakrishnan. Gene transfer by F′ strains of Escherichia coli. IV. The effect of a chromosomal deletion on chromosome transfer. J. Bacteriol. 88:367–373. 1964.—Evidence is presented that in an F′ strain of Escherichia coli. AB1206, the chromosomal region corresponding to the merogenote of F14 is deleted. AB1206 differs from all other F′ strains in its stability and in its relative inability to transfer chromosomal markers under conditions in which transfer of the merogenote occurs at a frequency of 100%. Its stability can be accounted for by the fact that the loss of the F merogenote, which contains approximately 10% of the genetic information of the cell, would be a lethal event. The failure of AB1206 to transfer chromosomal markers, except at very low frequencies, can be explained in terms of the crossover model proposed to explain chromosome transfer by F′ strains, since the homologous chromosomal region is missing in this strain. The conclusion that the chromosomal region is deleted is strengthened by the finding that the different enzymes coded for by the genes on F14 are present in AB1206 at the level found in haploid strains, but have double this activity in homozygous diploids carrying F14.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADELBERG E. A., BURNS S. N. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. doi: 10.1128/jb.79.3.321-330.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ARMSTRONG F. B., WAGNER R. P. Biosynthesis of valine and isoleucine. IV. alpha-Hydroxy-beta-keto acid reductoisomerase of Salmonella. J Biol Chem. 1961 Jul;236:2027–2032. [PubMed] [Google Scholar]
  3. GAREN A., GAREN S. Complementation in vivo between structural mutants of alkaline phosphatase from E. coli. J Mol Biol. 1963 Jul;7:13–22. doi: 10.1016/s0022-2836(63)80015-7. [DOI] [PubMed] [Google Scholar]
  4. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  5. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  6. MYERS J. W. Dihydroxy acid dehydrase: an enzyme involved in the biosynthesis of isoleucine and valine. J Biol Chem. 1961 May;236:1414–1418. [PubMed] [Google Scholar]
  7. PITTARD J., ADELBERG E. A. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. II. INTERACTION BETWEEN F-MEROGENOTE AND CHROMOSOME DURING TRANSFER. J Bacteriol. 1963 Jun;85:1402–1408. doi: 10.1128/jb.85.6.1402-1408.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PITTARD J., ADELBERG E. A. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. III. AN ANALYSIS OF THE RECOMBINATION EVENTS OCCURRING IN THE F' MALE AND IN THE ZYGOTES. Genetics. 1964 Jun;49:995–1007. doi: 10.1093/genetics/49.6.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PITTARD J., LOUTIT J. S., ADELBERG E. A. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. I. DELAY IN INITIATION OF CHROMOSOME TRANSFER. J Bacteriol. 1963 Jun;85:1394–1401. doi: 10.1128/jb.85.6.1394-1401.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. RADHAKRISHANAN A. N., SNELL E. E. Biosynthesis of valine and isoleucine. 2. Formation of alpha-acetolactate and alpha-aceto-alpha-hydroxybutyrate in Neurospora crassa and Escherichia coli. J Biol Chem. 1960 Aug;235:2316–2321. [PubMed] [Google Scholar]
  11. RUDMAN D., MEISTER A. Transamination in Escherichia coli. J Biol Chem. 1953 Feb;200(2):591–604. [PubMed] [Google Scholar]
  12. UMBARGER H. E., BROWN B. Threonine deamination in Escherichia coli. II. Evidence for two L-threonine deaminases. J Bacteriol. 1957 Jan;73(1):105–112. doi: 10.1128/jb.73.1.105-112.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

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