Table II.

Ki values of the barley cystatins against cathepsin L-like proteases from commercial origin (papain) and from barley expressed as recombinant proteins in E. coli cultures (HvPap-4, HvPap-6, HvPap-10, and HvPap-16)

No inhibitory activity (ni) was observed at 5 mm concentration of the cystatin.

Cystatin	Ki
	Papain	HvPap-4	HvPap-6	HvPap-10	HvPap-16
	m
HvCPI-1	2.0 × 10−8	1.2 × 10−7	1.0 × 10−7	4.1 × 10−9	1.2 × 10−7
HvCPI-2	5.1 × 10−8	1.0 × 10−8	2.3 × 10−9	3.4 × 10−10	6.2 × 10−9
HvCPI-3	4.0 × 10−8	ni	1.6 × 10−8	1.9 × 10−6	6.4 × 10−7
HvCPI-4	2.2 × 10−7	1.4 × 10−7	2.2 × 10−8	7.2 × 10−8	4.0 × 10−7
HvCPI-5	6.3 × 10−8	8.1 × 10−9	6.6 × 10−8	1.3 × 10−8	8.2 × 10−8
HvCPI-6	1.7 × 10−9	1.3 × 10−7	9.4 × 10−8	2.8 × 10−9	2.5 × 10−9
HvCPI-7	ni	ni	ni	ni	ni
HvCPI-8	1.0 × 10−8	4.9 × 10−8	9.3 × 10−8	4.3 × 10−9	2.9 × 10−8
HvCPI-9	1.4 × 10−9	4.7 × 10−8	1.3 × 10−8	2.1 × 10−7	1.3 × 10−7
HvCPI-10	3.3 × 10−7	6.9 × 10−7	3.3 × 10−6	ni	1.0 × 10−5
HvCPI-11	8.2 × 10−8	7.0 × 10−7	2.9 × 10−7	7.0 × 10−7	4.3 × 10−6
HvCPI-12	ni	ni	ni	ni	ni
HvCPI-13	1.4 × 10−6	1.9 × 10−6	1.2 × 10−6	ni	ni