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. 2009 Nov;151(3):1513–1530. doi: 10.1104/pp.109.142059

Figure 4.

Figure 4.

Light induces expression of VvMYBF1 and VvFLS1 and subsequent flavonol synthesis in Chardonnay cells. Transcript levels were determined by qPCR and are shown relative to VvUbiquitin1 (TC32075) expression. Each data point represents the mean of three replicate PCRs with error bars indicating se. A, VvMYBF1 expression. B, VvFLS1 transcript levels in Chardonnay cell samples exposed to light. C, Accumulation of total flavonols in Chardonnay cell samples exposed to light (gray bars) and kept in the dark (black bars) determined by HPLC analysis. D, Positions of putative cis-regulatory elements in the promoters of VvMYBF1 and VvFLS1 from Shiraz, Pinot Noir, and Chardonnay relative to the transcriptional start codon. The putative transcriptional start sites are referred to as positions +1, while the translational start codons are marked by the bent arrows. Numbers above the boxes indicate the distance from the transcriptional start. ACS, ACGT-containing sequence similar to ACE in the Arabidopsis CHS promoter (accession no. S000355); MRS, MYB recognition sequence similar to MRE in the Arabidopsis CHS promoter (S000356); RRS, R response sequence similar to RRE in the Arabidopsis CHS promoter (S000407); IBOXCORE element (S000199), LREBOXIPCCHS1 consensus sequence (S000302), MYBCORE (S000176).