Figure 5.

Loss-of-function analysis suggested that SPL/NZZ was involved in regulating stamen identity. A, A wild-type flower showing petals in whorl 2 and stamens in whorl 3. B, An spl flower exhibiting normal petals in whorl 2 and sterile stamens (no pollen released) in whorl 3. C, An ag-4 flower showing normal petals in whorl 2 and normal stamens in whorl 3. D, An spl ag-4 double mutant flower displaying normal petals in whorl 2 and stamens resembling those of spl inward whorl 2. E, SEM image exhibiting a wild-type petal. F and G, Low-magnification (F) and high-magnification (G) views of wild-type petal epidermal cells showing uniform size and cone shape. H, A wild-type anther. I, An spl anther showing flattened shape and undeveloped lobes. J, High-magnification view of epidermal cells of the spl anther, which are the same as wild-type anther epidermal cells (Fig. 2D). K, An ag-4 anther is similar to the wild-type anther. L, An spl ag-4 double mutant petal-like (petaloid) organ from whorl 3 showing no developed anther lobes. M, High-magnification view of epidermal cells of the organ shown in L showing the resemblance to wild-type petal epidermal cells in F. N, An spl ag-4 double mutant petal-like organ from whorl 3. O, High-magnification view of epidermal cells of the organ shown in N, which is similar to wild-type petal epidermal cells in G. Bars = 0.5 mm (A–D), 200 μm (E), 50 μm (L and N), 20 μm (H, I, and K), 10 μm (F and M), and 5 μm (G, J, and O).