Skip to main content
. 2009 Nov;151(3):1401–1411. doi: 10.1104/pp.109.145896

Figure 6.

Figure 6.

Ectopic expression and overexpression of SPL/NZZ altered the expression of genes required for stamen identity in rosette leaves and flowers. A, RT-PCR results showing greatly increased expression of SPL/NZZ, AG, and SEP3 in both Pro35S:SPL/NZZ and spl-D rosette leaves. There was no detectable change of CLF expression. B, RT-PCR results showing that expression of SPL/NZZ, AG, and SEP3 was increased in Pro35S:SPL/NZZ and spl-D flowers as well as Pro35S:SPL/NZZ sepals and petals. The expression of AP2 was slightly decreased, while the expression of AP1 and AP3 seemed unchanged. C, Quantitative real-time RT-PCR results showing the increased expression of AG in rosette leaves, flowers, sepals, and petals of Pro35S:SPL/NZZ plants. D, Quantitative real-time RT-PCR results showing the increased expression of SEP3 in rosette leaves, flowers, sepals, and petals of Pro35S:SPL/NZZ plants. E, Quantitative real-time RT-PCR results illustrating the decreased expression of AP2 in rosette leaves, flowers, sepals, and petals of Pro35S:SPL/NZZ plants. F, Semiquantitative RT-PCR results showing the increased expression of SEP3 in Pro35S:SPL/NZZ-GR ag-1 inflorescences after both DEX and DEX + Cyc treatments for 4 h. The LIPASE gene was used as a control. The transcripts in wild-type rosette leaves in C to E were used as a standard for normalization. Asterisks indicate that the difference is significant (P < 0.01 or P < 0.05). REL, Relative expression level; S & P, sepal and petal; 35S:SPL, Pro35S:SPL/NZZ.