Fig. 2.

Expression of Sp8/9 in Oncopeltus embryos. a Early germband elongation stage. Arrows point to expression extending from the thoracic limb buds into the lateral tissue of the thoracic segments posterior to the limb buds. b Mid-elongation germband stage. c Embryo approaching the final stages of germband elongation. d Fully elongated germband stage. e Retracted germband stage. f Magnification of a leg at the retracted germband stage showing the expression rings of Sp8/9 abutting the presumptive joint constrictions (arrowheads). All embryos are oriented with the anterior end to the left. Abbreviations: lr labrum, an antenna, md mandible, mx maxilla, lb labium, t1 prothoracic leg, t2 mesothoracic leg, t3 metathoracic leg