Figure 1.
Synthesis of the ROS/RNS sensor. a–b. Reaction of oxazine 1 with glutaric anhydride to generate the quenched ROS responsive intermediate with conjugatable handle for attachment to the dextran shell of the iron oxide nanoparticles. Nanoparticles are dual labeled with Alexa Fluor 488 to monitor particle location c–d, Relative fluorescence signal for each of the reactants and products. e–f, Blood half-life determination for the free oxazine dye and the ROS/RNS sensor.