Table 1. NOEs Observed between the AFB-α-FAPY Adduct and DNA Protonsa.
AFB-α-FAPY | 10-mer ds duplex | tetramer |
---|---|---|
H2α | C16 H1′, C16 H3′, C16 H2′′, C16 H4′, C16 H5, C16 H6, A17 H8 | |
H2β | A17 H8 | |
H3α | A17 H3′ | |
H3β | A17 H8 | |
H5 | A17 H2, T4 H6, T4 H4′ | T4 H2′, T4 H2′′ |
H6a | T4 CH3, T4 H6, X5 H8 | |
H8 | X5 CHO | X3 CHO |
H9a | T4 CH3, T4 H6, T4 H1′, X5 H8 | |
H9 | ||
-OCH3 | A17 H2, T4 H1′, T4 H2′, T4 H2′′, T4 H5′, T4 H3, X5 H3 | T4 H1′ |
NOEs involving adenine H2 protons were not included in structural refinement due to the long T1 relaxation times associated with these protons.