Fig. 2.

The Arp2/3 complex is enriched near cell membranes. (A-F) Embryos immunostained with ARX-5 antibodies (green). (A,B) Embryos at the 6- to 8-cell stage. ARX-5 appears enriched near plasma membranes (arrowheads). In control arx-5 RNAi embryos, enrichment near plasma membranes is reduced or absent. (C-F) Lateral views (C,D) and ventral views (E,F) of gastrulation-stage embryos also revealed ARX-5 localization near membranes (arrowheads). ARX-5 was similarly enriched at borders between MSxx and Ea cells (arrow). Cortical staining was absent in arx-5 RNAi embryos. Antibodies to P-granules were used to confirm permeabilization of embryos to immunostaining reagents (red). Nuclei are stained with DAPI (blue).