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Detection of PSA by immunoRCA in a microspot assay. (A) Fluorescent microscope images of immunoRCA signals obtained with different concentrations of human PSA captured on microspots of anti-human PSA Ab. Concentrations: (Upper Left) 5 pg/ml PSA; (Lower Left) 0.5 pg/ml PSA; (Upper Right) 0.1 pg/ml PSA; (Lower Right) 0 pg/ml PSA. (B) Fluorescence in microscope images was quantitated as described in Methods and plotted versus PSA concentrations incubated on microspots.
