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. Author manuscript; available in PMC: 2009 Nov 4.
Published in final edited form as: Chem Res Toxicol. 2008 Nov;21(11):2073–2081. doi: 10.1021/tx800140y

Figure 6. Electronic absorption spectra of cytochrome c oxidase derivatives showing displacement of CN- by both NO and CO.

Figure 6

Samples were prepared in 100 mM aqueous potassium phosphate buffer, pH 7.4, 0.05 % lauryl maltoside, 22°C, 1.00 cm pathlengths. Broken trace: partially-reduced cyanide adduct, 12 μM in enzyme, 0.2 mM in KCN, ~1 mM in Na2S2O4; dotted trace: partially-reduced cyanide adduct plus CO, 12 μM in enzyme, 0.2 mM in KCN, ~1 mM in Na2S2O4, ~1 mM (1.0 atm) CO; solid trace: partially-reduced cyanide adduct plus NO, 12 μM in enzyme, 0.2 mM in KCN, ~1 mM in Na2S2O4, 1.9 mM (1.0 atm) NO.