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. Author manuscript; available in PMC: 2009 Nov 4.

Published in final edited form as: Chem Res Toxicol. 2008 Nov;21(11):2073–2081. doi: 10.1021/tx800140y

Samples were prepared in 100 mM aqueous potassium phosphate buffer, pH 7.4, 22°C, 1.00 cm pathlengths. A. Electronic absorption spectral changes (500-620 nm range) over time (~1 hr) of 14 μM metHbCN plus 0.95 mM (0.5 atm) NO. B. Linear dependence of the rate of reaction on the NO concentration.

Samples were prepared in 100 mM aqueous potassium phosphate buffer, pH 7.4, 22°C, 1.00 cm pathlengths. A. Electronic absorption spectral changes (500-620 nm range) over time (~1 hr) of 14 μM metHbCN plus 0.95 mM (0.5 atm) NO. B. Linear dependence of the rate of reaction on the NO concentration.