Figure 2. TGFβ signalling in MTLn3E cells.
A: Assay showing changes in CAGA12-luciferase reporter activity in MTLn3E cells in response to 2ng/ml TGFβ1 +/−10μM SB431542 (an inhibitor of the TGFβ type I receptor Alk 5 - also inhibits Alk4&7). B: Soft agar assays were set up using MTLn3E cells that had been cultured in control media (+ vehicle) or with 2ng/ml TGFβ1 or 10μM SB431542 for 24 hours. Average of two experiments (error bars represent half range). C: MTLn3E tumour stained for pSmad3 (green) and DAPI (blue), tumour margin is in top right corner.