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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Nat Cell Biol. 2009 Oct 18;11(11):1287–1296. doi: 10.1038/ncb1973

Figure 5. TGFβ requires Smad4 to switch cells to single cell motility.

Figure 5

A: Time course of switch to single cell motility induced by 2ng/ml TGFβ. Scattering of colonies was used as a readout of single cell motility. B: F-actin and β-catenin staining of MTLn3E cells seeded at low density in the presence or absence of TGFβ 2ng/ml for 40 hours (see also Movie 9). C: Graph shows the cell scattering 24hr after addition of 2ng/ml TGFβ in control and Smad1, 2, 3, 4, 5, 1&5 or 2&3 siRNA smartpool transfected cells (average of two or three experiments, * p<0.05, ** p<0.01 student t-test). D: Western blot showing the efficiency of Smad4 depletion using 4 different siRNA sequences. Graph shows the cell scattering 24hr after addition of 2ng/ml TGFβ following transfection of three different Smad4 siRNA sequences (averages and standard deviations from one representative experiment of three are shown, * p<0.05). E: β-catenin staining of MTLn3E cells in control and Smad4 siRNA transfected cells 24hr after addition of 2ng/ml TGFβ.

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