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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Nat Cell Biol. 2009 Oct 18;11(11):1287–1296. doi: 10.1038/ncb1973

Figure 6. Roles of multiple TGFβ target genes in the switch to single cell motility.

Figure 6

A: Graph shows the cell scattering 24hr after addition of 2ng/ml TGFβ in mock, control, RhoA, RhoC, RhoA+C, MPRIP, FARP1, RhoQ, NEDD9, FXYD5, CTGF, EGFR, PAI-1, c-Jun and JunB siRNA smartpool transfected cells (average of 2-4 experiments, * p<0.05, ** p<0.01 student t-test). B: Graph shows the cell scattering 24hr after addition of 2ng/ml TGFβ in control, 10 μM SB431542, 10 μM AG1478, 10 μM SP600125, and 10 μM Y27632 treated cells. C: β-catenin staining of MTLn3E cells in mock, control, RhoA+C, MPRIP, FARP1, NEDD9, CTGF, EGFR, and c-Jun siRNA transfected cells 24hr after addition of 2ng/ml TGFβ. D: Analysis of single ‘amoeboid’ cell motility: mock, control, RhoA+C, MPRIP, FARP1, NEDD9, CTGF, EGFR, and c-Jun siRNA transfected MTLn3E cells were plated as single cells on deformable collagen gels and the distance moved over 90 minutes was measured. Box and whisker plots show mean, quartiles and 10 and 90 percentiles (average of 2-4 experiments, * p<0.05, ** p<0.01 student t-test) E: F-actin staining of MTLn3E cells in mock, control, RhoA+C, MPRIP, FARP1, NEDD9, CTGF, EGFR, and c-Jun siRNA transfected cells plated as single cells on deformable collagen gels.

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