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. 2009 Aug 25;18(23):4513–4520. doi: 10.1093/hmg/ddp411

Figure 2.

Figure 2.

HS4-1512C selectively binds Oct-1. Radiolabeled HS4 probes carrying an A or a C at position –1512 (A) were incubated with nuclear extracts from in vitro polarized murine Th2 cells (mTH2 NE) (B and C) or human Th2 cells (hTh2 NE) (D) cultured for 3 h in the presence or absence of PMA (20 ng/ml) and ionomycin (1 µM) (P/I) or in vitro translated recombinant Oct-1 (rOct-1) (C). EMSA probes and experimental conditions are noted below the gels. Competitors (100-fold molar excess) and supershifting antibodies added to each reaction are noted above the relevant lanes. A Gata3 consensus oligonucleotide and IgG1κ or an anti-Gata3 antibody were used as unrelated controls. Ns: non-specific.