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. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: J Med Chem. 2009 Jan 22;52(2):358–368. doi: 10.1021/jm801052f

Figure 2.

Figure 2

(A) Changes in fluorescence intensity of FAP-PPB and 2D-FAP-PPB upon interactions with human FAP and mFAP (50:1 molar ratio) in a TRIS buffer solution at 37 °C. (B) HPLC profile of FAP-PPB after 1-hour incubation with mFAP. (C) UV-Vis spectra for the corresponding portions. (D) Variation of the initial cleavage rate of FAP-PPB by mFAP (2 μg) (as shown by the increase in fluorescence intensity at 665 nm) with increasing concentration of FAP-PPB. The estimated Km and Vmax values are indicated and the error bars represent standard deviations from the mean from triplicate measurements.