Fig. 2.

Multiple alignment of the amino acid sequences of AO090012000706, AO090701000220, AO090103000026 (CpI), and three known serine-type carboxypeptidases: S. cerevisiae carboxypeptidase Y (CPY; NP_014026), Aspergillus phoenicis CpdS (P52719), and P. janthinellum carboxypeptidase S1 (CPD-S1; AAB28596). The sequence of CPD-S1 contains no preprosequence. The conserved substrate binding motif is indicated by the line (I), and catalytic motifs harboring the amino acid of the catalytic triad (filled lozenge) are marked II–IV. S₁ binding sites are shown by filled reverse triangles, S₁′ binding sites by filled circles, and completely conserved residues by asterisks