Fig. 5.

Effects of protease inhibitors on the carboxypeptidase activities of recombinants OcpA, OcpB, and CpI. PMSF, E64, MIA, pepstatin A, and EDTA were added to the reaction mixture at final concentrations of 1, 0.1 mM, 3 μg/ml, 1, and 1 mM, respectively. The activities for Z-Glu-Tyr of the enzymes with each inhibitor were measured at pH 3.7 at 30°C. Reaction mixtures containing water or dimethyl sulfoxide instead of each inhibitor were used as reference. Relative activities were quantified as the percentage activity of each reference. Open bar recombinant OcpA; filled bar recombinant OcpB; hatched bar recombinant CpI