Table 1.
Characteristic chemical shifts (δ in ppm) and coupling constants (J) of some compounds detected in the 1H-NMR and J-resolved 1H-NMR spectra (CH3OH-d 4-KH2PO4 in D2O, pH 6.0, 1:1) of control- and SA-treated cells of Catharanthus roseus suspension culture
| Compound | Chemical shift (ppm) and coupling constant (Hz) |
|---|---|
| Leucine | δ 0.97 (d, J = 6.5 Hz); δ 0.98 (d, J = 6.7 Hz) |
| Isoleucine | δ 0.95 (t, J = 7.5 Hz); δ 1.02 (d, J = 6.8 Hz) |
| Valine | δ 1.00 (H-γ, d, J = 7.0 Hz); δ 1.05 (H-γ′, d, J = 7.0 Hz) |
| Threonine | δ 1.33 (H-γ, d, J = 6.6 Hz) |
| Alanine | δ 1.48 (H-β, d, J = 7.2 Hz) |
| Glutamic acid | δ 2.46 (H-γ, m), δ 2.13 (H-β, m) |
| Aspartic acid | δ 2.82 (H-, dd, J 1 = 8.1 Hz, J 2 = 17.0 Hz); |
| δ 2.95 (H-, dd, J 1 = 3.8 Hz, J 2 = 16.8 Hz); | |
| δ 3.94 (H-, dd, J 1 = 3.8 Hz, J 2 = 8.4 Hz) | |
| Glucose | δ 5.18 (α-form, H-1, d, J = 3.8 Hz), δ 4.58 (β-form, H-1, d, J = 7.8 Hz), |
| Sucrose | δ 5.40 (H-1, d, J = 3.8 Hz); δ 4.17 (H-1’, d, J = 8.6 Hz) |
| Fumaric acid | δ 6.54 (s) |
| Phenylalanine | δ 7.36 (m) |
| Salicylic acid (2-hydroxybenzoic acid) | δ 6.88 (H-3, d, J = 8 Hz); δ 6.90 (H-5, t, J = 7.5 Hz); |
| δ 7.37 (H-4, t, J = 7.5 Hz); δ 7.81 (H-6, d, J = 8 Hz) | |
| Gentisic acid glucoside (2,5-dihydroxybenzoic acid glucoside) | δ 6.83 (H-3, d, J = 9 Hz); δ 7.17 (H-4, dd, J 1 = 9 Hz, J 2 = 3 Hz); δ 7.56 (H-6, d, J = 3 Hz); δ 4.88 (H-1’, d, J = 7.5 Hz) |
s Singlet, d doublet, t triplet, m multiplet