Table 1. CoA-dependent α-ketoglutarate: MV oxidoreductase activity in M. tuberculosis, M. bovis and M. smegmatis.
Strain | nmol MVred min−1 mg−1 protein | ||
KG | NADH | Pyruvate | |
M. tuberculosis mc27000 (wild type) | 1.0±0.1 | 1.7±0.1 | <0.01 |
M. tuberculosis mc27010 (ΔkorAB) | <0.01 | 1.9±0.1 | NM* |
M. tuberculosis mc27011 (ΔkorAB - complemented) | 1.3±0.1 | 1.9±0.1 | NM |
M. tuberculosis H37Ra | 0.68±0.15 | NM | NM |
M. bovis BCG | 1.6±0.2 | NM | <0.01 |
M. smegmatis mc2155 | 1.7±0.2 | 2.9±0.2 | 0.59±0.02 (0.32)† |
*: Not measured.
†: A fraction of pyruvate: MV oxidoreductase activity from M. smegmatis mc2155 was CoA-independent (shown in parentheses).