Table 2. Average K2P distance and diagnostic sites for the eight species comprising the genus Thunnus.

Species (sample size)	Albacore	Bigeye tuna	Blackfin tuna	Longtail tuna	Northern bluefin tuna	Pacific bluefin tuna	Southern bluefin tuna	Yellowfin tuna
	Average K2P distance
Albacore (12)	0.00172	0.02026	0.01574	0.01877	0.00313	0.01924	0.01773	0.01755
Bigeye tuna (15)	268, 286, 313, 358, 400, 478, 484	0.00313	0.00807	0.00666	0.01888	0.0106	0.0108	0.00509
Blackfin tuna (9)	313, 358, 400, 478, 484, 535	268, 286, 400, 484, 508	0.00386	0.00721	0.01616	0.01113	0.01246	0.0052
Longtail tuna (5)	262, 268, 313, 358, 478, 484, 535	262, 286, 400	262, 286, 400	0.00123	0.01685	0.00947	0.00988	0.00353
Northern bluefin tuna (13)	268, 313, 337, 409, 478, 484, 535	271, 286, 337, 358, 400, 409	268, 271, 358, 400, 409, 484	262, 271, 337, 358, 400, 409, 484	0.00239	0.01833	0.01494	0.01651
Pacific bluefin tuna (5)	475	268, 286, 313, 358, 400, 475, 478, 484, 535	313, 358, 400, 475, 478, 484, 535	262, 268, 313, 358, 475, 478, 484, 535	268, 271, 313, 337, 409, 475, 478, 484, 535	0.00046	0.00955	0.00774
Southern bluefin tuna (5)	268, 313, 337, 478, 484, 508, 535	286, 337,358, 400, 484, 508	268, 337, 358, 400, 508	262, 337, 358, 484, 508	271, 337, 409, 484, 508	268, 313, 337, 475, 478, 484, 508, 535	0.00276	0.00867
Yellowfin tuna (23)	268, 313, 358, 475, 478, 484, 535	#	268, 358, 400, 475, 484	262, 358, 475	271, 337, 358, 409, 475	268, 313, 358, 475, 478, 484, 535	337, 358, 475, 484, 508	0.00195
	Diagnostic sites

# Private compound diagnostic. The predominant nucelotide sequence for T. albacares is C262 C268 C271 C286 A313 C337 G358 T400 A409 T475 T478 G484 A508 C535. The majority of T. obesus (n = 10) can be differentiated by T286 G400. One T. obesus (DQ835865) is differentiated by A286, two are differentiated by C400 (DQ835861, DQ835862), and two by G400.