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. 2008 Nov 21;94(2):213–219. doi: 10.1113/expphysiol.2008.045112

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© 2009 The Physiological Society

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Control and ligated submandibular glands Nerve staining of collagenase-digested cell clumps from control (A) and 4-week-ligated glands (B). Cholinesterase nerve staining indicates that parasympathetic nerves (arrow in A) are still attached following collagenase digestion. Alcian Blue/Periodic Acid Schiff's staining of tissue sections reveals blue acinar cells (arrows) and pink granular ducts (*) in normal glands (C) and an almost complete loss of secretory granules in 4-week-ligated glands (D). Scale bar represents 30 μm.